Home
Programs
Center for Laboratory Sciences
Infectious Diseases Laboratory
Viral and Rickettsial Disease Laboratory
VRDL Test Page - Mumps virus PCR

Viral And rickettsial Disease Laboratory

Test Order
Mumps Virus Detection - PCR

Information Category	Information Detail
Pre-Approval	None
Supplemental Information Requested	Requested: Travel history, disease onset date, symptoms, mumps vaccination history, contacts to known case(s)
Submittal Form	Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form (PDF) or a form generated in the VRDL Lab Web Portal.
Methodology	Real-time RT-PCR (LDT) (Diagnostic)
Reflex Testing	Genotyping
Acceptable Specimen Type(s) and Collection Method	Human specimens: Buccal Swab (preferred) or Throat (Oropharyngeal) Swab in sterile collection tube with liquid viral or universal transport media Urine: processed urine preferred Swabs must be a Dacron or synthetic tip with aluminum or plastic shaft Other respiratory specimen types may be considered. Please contact the Medical and Epidemiology Liaisons for consultation. To process urine, centrifuge urine sample (10-50 mL) at 500–600 x g for 5–10 min at 2–8^o C. Discard supernatant and resuspend cell sediment in 2–3 mL of viral transport media.
Minimum Volume Requested	Buccal swabs: 2–3 mL of Universal or Viral Transport Medium Urine, processed: Pellet resuspended in 2–3 mL of Universal or Viral Transport Medium Urine, unprocessed: 10–50 mL.
Transport Medium (if using)	Swabs or Urine pellet: 2–3 mL of Universal or Viral Transport Medium Urine, unprocessed: Not applicable
Specimen Labeling	Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions	Swabs: freeze or refrigerate specimens promptly after collection. Urine pellets: freeze or refrigerate specimens promptly after collection. Unprocessed urine: refrigerate specimens promptly after collection. Do not freeze unprocessed urine. Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
Shipping Instructions	Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment. Ship specimens and a hard copy of the completed submittal forms to: CDPH VRDL ATTN: Specimen Receiving 850 Marina Bay Parkway Richmond, CA 94804 Phone: 510-307-8585
Turnaround Time	2 Business days
Limitations	Negative results do not preclude the possibility of infection
Additional Information	Specimens should be collected as close to onset as possible.
VRDL Points of Contact	Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov

Page Last Updated :