| Pre-Approval | None |
| Supplemental Information Requested | Requested: Travel history, disease onset date, symptoms, mumps vaccination history, contacts to known case(s)
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| Submittal Form | Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form (PDF) or a form generated in the VRDL Lab Web Portal.
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| Methodology | Sequence based genotyping (LDT) (Surveillance use only)
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| Reflex Testing | May be reflexed to whole genome sequencing
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| Acceptable Specimen Type(s) and Collection Method | Human specimens:
- Buccal Swab (preferred) or Throat (Oropharyngeal) Swab in sterile collection tube with liquid viral or universal transport media
- Urine: processed urine preferred
- Mumps-positive nucleic acid extracts
Swabs must be a Dacron or synthetic tip with aluminum or plastic shaft
Other respiratory specimen types may be considered. Please contact the Medical and Epidemiology Liaisons for consultation.
To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2–8o C. Discard supernatant and resuspend cell sediment in 2–3 mL of viral transport media.
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| Minimum Volume Requested | - Buccal swabs: 2–3 mL of Universal or Viral Transport Medium
- Urine, processed: Pellet resuspended in 2–3 mL of Universal or Viral Transport Medium
- Urine, unprocessed: 10-50 mL
- Nucleic acid extracts: 50 µL
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Transport Medium (if using)
| - Swabs or Urine pellet: 2–3 mL of Universal or Viral Transport Medium
- Urine, unprocessed: Not applicable
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| Specimen Labeling | Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth. |
| Storage & Preservation of Specimen / Shipping Conditions | - Swabs: freeze or refrigerate specimens promptly after collection.
- Urine pellets: freeze or refrigerate specimens promptly after collection.
- Unprocessed urine: refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
- Nucleic Acid Extracts: freeze prior to shipment
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice. |
| Shipping Instructions | Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment. Ship specimens and a hard copy of the completed submittal forms to: CDPH VRDL
ATTN: Specimen Receiving
850 Marina Bay Parkway
Richmond, CA 94804
Phone: 510-307-8585
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| Turnaround Time | 10 Business days |
| Limitations | Negative results do not preclude the possibility of infection
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| Additional Information | Specimens should be collected as close to onset as possible. |
VRDL Points of Contact
| Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov
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