Skip Navigation LinksMeasles-Virus-PCR

VIRAL AND RICKETTSIAL DISEASE LABORATORY

 Test Order

Measles Virus (Rubeola) Detection - PCR

​Information Category
​Information Detail
Pre-Approval

Not Applicable

​Supplemental Information Requested
Req​uested: Travel history, rash onset date, symptoms, measles vaccination history, contacts to known case(s)
​Submittal Form
Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form​​ (PDF) or a form generated in the VRDL Lab Web Portal.​
Methodology 
Real-Time RT-PCR (LDT) (Diagnostic)
Reflex Testing
Genotyping
​Acceptable Specimen Type(s) and Collection Method
Human specimens: 
  • Throat (preferred) and/or NP swab in sterile collection tube with liquid viral transport media
  • Urine: Processed urine preferred
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft. 

To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8°C.  Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
​Minimum Volume Requested
  • Respiratory swab: 2–3 mL of Universal or Viral Transport Medium
  • Urine, unprocessed: 10-50 mL  
  • Urine, processed: Pellet resuspended in 2–3 mL of Universal or Viral Transport Medium 
​Transport Medium (if using)
For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium 
​Specimen Labeling
​Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth. 
​Storage & Preservation of Specimen / Shipping Conditions
  • Respiratory swabs and urine pellets: freeze or refrigerate specimens promptly after collection.
  • For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
​Shipping Instructions
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.  
Ship specimens and a hard copy of the completed submittal forms to: 

CDPH VRDL  
ATTN: Specimen Receiving 
850 Marina Bay Parkway  
Richmond, CA 94804  
Phone: 510-307-8585 
Turnaround Time
2 Business Days
Limitations
​Negative results do not preclude the possibility of infection
​Additional Information
Specimens should be collected as close to rash onset as possible and preferably within 14 days of rash onset. 
​VRDL Points of Contact

Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov​

​​
Page Last Updated :