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VRDL Test Page - Dengue Virus Serology

VIRAL AND RICKETTSIAL DISEASE LABORATORY

Test Order
Dengue Virus - Serology

Information Category	Information Detail
Pre-Approval	None
Supplemental Information Requested	Requested: Travel history, illness onset date
Submittal Form	Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form (PDF) or a form generated in the VRDL Lab Web Portal.
Methodology (Commercial Test Name or Laboratory Developed Test (LDT))	EIA: IgM (Commercial Test)(Diagnostic)
Reflex Testing	Flavivirus Plaque Reduction Neutralization Test
Acceptable Specimen Type(s) and Collection Method	Human specimens: Serum or whole blood: red top or red/gray top serum separator tubes
Minimum Volume Requested	Serum: 1 mL Whole blood in serum separator tubes: 5 mL
Transport Medium (if using)	Not Applicable
Specimen Labeling	Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions	Serum: freeze or refrigerate specimens after collection. Whole blood: refrigerate specimens promptly after collection. Do not freeze whole blood. Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
Shipping Instructions	Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment. Ship specimens and a hard copy of the completed submittal forms to: CDPH VRDL ATTN: Specimen Receiving 850 Marina Bay Parkway Richmond, CA 94804 Phone: 510-307-8585
Turnaround Time	10 Business Days
Limitations	Serologic assays do not distinguish between dengue serotypes. Positive result does not confirm infection, supplemental testing is required. Negative result does not preclude the possibility of infection. Flavivirus antibodies are often cross-reactive. Cross-reactivity with Malaria IgM has not been evaluated with the DENV Detect IgM Capture ELISA. Results from immunosuppressed patients must be interpreted with caution. High cholesterol levels (>300 mg/dL) appear to give variable results and may affect the DENRA OD values. High triglyceride levels (>3000 mg/dL) appeared to exhibit a slight effect of raising the ISRs of low positive sera. Hemoglobin (>1600 mg/dL) appears to affect some serum samples by lowering the ISRs.
Additional Information	A convalescent specimen is recommended to help determine recency of infection. When submitted separately, be sure to identify the previously submitted specimen by the VRDL accession number so that the acute and convalescent specimens may be linked, tested to endpoint and interpreted as a pair.
VRDL Points of Contact	Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov

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