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Test Order
Dengue Virus Detection - PCR 

Information CategoryInformation Detail
Supplemental Information Requested
Requested: Travel history, illness onset date, collection date
Submittal FormSpecimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form​​ (PDF) or a form generated in the VRDL Lab Web Portal.
Methodology (Commercial Test Name or Laboratory Developed Test (LDT))CDC EUA Trioplex Real-time RT-PCR Assay (Diagnostic)
Reflex TestingNone
Acceptable Specimen Type(s) and Collection Method

Human specimens:

  • Serum or whole blood: red top or red/gray top serum separator tubes
  • Whole blood: EDTA (lavender top) tubes
  • CSF: sterile collection tubes
Minimum Volume Requested
  • Serum: 1 mL
  • Whole blood in serum separator tubes: 5 mL
  • Whole blood in EDTA whole blood tubes: 5 mL
  • CSF: 1 mL
Transport Medium (if using)Not Applicable
Specimen LabelingEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions
  • Serum and CSF: freeze or refrigerate specimens after collection.
  • Whole blood: refrigerate specimens promptly after collection. Do not freeze whole blood.

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

Shipping Instructions


Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.

Ship specimens and a hard copy of the completed submittal forms to:    


ATTN: Specimen Receiving

850 Marina Bay Parkway

Richmond, CA 94804

Phone: 510-307-8585

Turnaround Time10 Business Days
LimitationsNegative result does not preclude the possibility of infection.
Additional InformationSpecimens should be collected as close to onset as possible.
VRDL Points of ContactMedical and Epidemiology Liaisons: (510) 307-8585 or

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