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microbial diseases laboratory branch

Tā€‹ā€‹esā€‹ā€‹t Nameā€‹

Fungal Identification: Moldā€‹


Testā€‹ā€‹ Mā€‹ā€‹ā€‹ā€‹eā€‹ā€‹tā€‹hā€‹oā€‹ā€‹ā€‹dā€‹ā€‹ā€‹ologyā€‹ā€‹ ā€‹ā€‹ā€‹ā€‹Identification of mold species by MALDI-TOF mass spectrometry and/or fungal ITS sequencing

Reflex Testing
Cā€‹ā€‹riteria

ā€‹ā€‹Reflex to fungal ITS sequencing if species ID is not resolved by MALDI-TOF. May reflex send-out to CDC if ID is not resolved by ITS sequencing. 

Pre-Aā€‹ā€‹pproval Requiredā€‹

Nā€‹o

Sā€‹uppā€‹lemental
Inforā€‹mation and Required Form(s)ā€‹

ā€‹Barcode submā€‹ittal form 448 ā€œFungus Culture for Identificationā€‹ā€‹ā€‹ā€ or instructions for using the MDL Lab Web Portal (LWPā€‹) can be found at: MDL Submission Instructions and Formsā€‹.ā€‹ā€‹

ā€‹ā€‹ā€‹Instructions on completing the form at MDL Form 448ā€‹

ā€‹Required Specimen Type(s)

ā€‹Pure culture on solid media from human source only. ā€‹ā€‹

Minimum Volume Required Not Applicable 

Specimen Stability

 

Room Temperature: acceptable ā€‹

Refrigerated: acceptable 

Frozen: DO NOT freeze ā€‹

ā€‹Rejection Criteria

 

  • Samples with insufficient or conflicting labellingā€‹ā€‹
  • Leaking samples
  • ā€‹ā€‹Fungal cultures sent on petri plates
  • ā€‹Nonviable cultures
  • ā€‹Mixed cultures
  • Specimen/sample types not meeting acceptance criteria

Storage/Transport Conditions ā€‹ā€‹ā€‹ā€‹

ā€‹Grow isolate under appropriate conditions and verify purity prior to shipping.

Isolates may be stored under refrigeration or at ambient temperature prior to shipping.

Transport Medium,ā€‹
if applicable

DO NOT submit mold isolates on petri plates due to safety concerns. Solid media in tube or flask with tightened screw cap and taped is preferable for submission. ā€‹ā€‹

Specimen Labeling Two identifiers, including patient full name, and at least one other identifier (specimen accession number, date of birth, medical record number).

ā€‹Shipping Instructions and Specimen Handling Requirements

 

 

 

 

ā€‹Ship cultures at ambient temperature (do not freeze). 

Follow the appropriate DOT/IATA approved shipping procedures. All the suspected Coccidioides cultures should be shipped as a Category A Infectious Substance, Affecting Humans (UN2814).

Ensure that culture isolates are sent in media flask or tubes containing a screw-top cap that is additionally sealed with parafilm or tape for additional protection against leaking.

Ship to:

California Departā€‹ment of Public Healthā€‹
Microbial Diseases Laboratory ā€‹
ATTN: Mycology Unitā€‹
ā€‹ā€‹C/O ā€‹Specimen Receiving Room B106ā€‹
850 Marina Bay Parkway,ā€‹
Richmond, CA  94804ā€‹

Specimen Collection Instructions, if applicable
Not Applicable
ā€‹Turnaround Time 14 days

Interferences & Limitations ā€‹ā€‹Not Applicableā€‹ā€‹

Reference Range Not Applicable

Additional Information Please contact the MDL Mycobacterial, Mycotic, and Parasitic Diseases Section for special consideration if any of the above criteria cannot be met.ā€‹

MDL Point ā€‹of Contact For questions regarding submissions, ā€‹please email CDPHMDLMMP@cdph.ca.gov or call the MMPDS Section at (510) 412-3926


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