Test Methodology
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Next Generation Sequencing
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Reflex Testing
Criteria
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Cyclospora specimens are routed to MDL for genotyping using Targeted Amplicon Deep Sequencing (TADS) method for public health surveillance and epidemiologic investigations only. Sequence data is uploaded to CDC for analysis. No reports are released by MDL.
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Pre-Approval Required
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Yes. Please contact MDL at MDL.Submissions@CDPH.ca.gov
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Supplemental Information and Required Form(s)
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Test Request Form for Cyclospora Targeted Amplicon Deep Sequencing (TADS). The submission form will be shared once shipment is approved.
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Required Specimen Type(s)
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Stool specimen submitted either in a Cary Blair or at least 3 aliquots in 2 mL sterile microcentrifuge tubes. Please secure top of container/ tubes with parafilm to avoid leakage during transport.
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Minimum Volume Required
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If submitted in microcentrifuge tube, 2 mL.
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Specimen Stability
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Refrigerated (2 – 8°C): up to 15 days
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Rejection Criteria
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- Specimens collected in
Formalin will be rejected.
- Leaking samples.
- Specimen other than stool such as fecal broth.
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Storage/Transport
Condition
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- Refrigerated: 2 – 8°C
- Frozen: -20°C or other (please indicate on Test Request Form)
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Transport Medium,
if applicable
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Cary-Blair modified media or VTM (Viral Transport Media)
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Specimen Labeling
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Containing patient identifiers: name, DOB, and collection date
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Shipping Instructions and Specimen Handling Requirements
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Specimen(s) should be shipped according to International Air Transport Association (IATA) and International Civil Aviation Organization (ICAO) regulations for biological specimens.
Ship to: California Department of Public Health
Microbial Diseases Laboratory
ATTN: Molecular Characterization Unit
850 Marina Bay Parkway, Specimen Receiving Room B106
Richmond, CA 94804
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Specimen Collection Instructions, if applicable
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Not Applicable
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Turnaround Time
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Not Applicable
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Interferences & Limitations
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None |
Reference Range
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Not Applicable |
Additional Information
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None
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MDL Point of Contact
| MDL - Molecular Characterization Unit
Phone: (510) 412-3940, M–F PST.
Email MDL.Submissions@CDPH.ca.gov
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