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VIRAL AND RICKETTSIAL DISEASE LABORATORY

 Test Order
Measles Virus (Rubeola) Identification- Genotyping

​Information Category
​Information Detail
Pre-Approval

Not Applicable

​Supplemental Information Requested
Requested: Travel history, rash onset date, symptoms, measles vaccination history, contacts to known case(s)
​Submittal Form
Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form​ (PDF) or a form generated in the VRDL Lab Web Portal.​
Methodology 
Sequence-based genotyping (LDT) (Surveillance use only)
Reflex Testing
May be reflexed to whole genome sequencing
​Acceptable Specimen Type(s) and Collection Method
Human specimens: 
  • Respiratory specimens: Throat (preferred) and/or NP swabs in sterile collection tube with liquid viral transport media
  • Urine: Processed urine preferred
  • Measles-positive nucleic acid extracts​
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft. 

To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8°C.  Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
​Minimum Volume Requested
  • Respiratory swab: 2–3 mL of Universal or Viral Transport Medium
  • Urine, unprocessed: 10-50 mL  
  • Urine, processed: Pellet resuspended in 2–3 mL of Universal or Viral Transport Medium 
  • Nucleic acid extracts: 25-50 µL
​Transport Medium (if using)
For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium 
​Specimen Labeling
​Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth. 
​Storage & Preservation of Specimen / Shipping Conditions
  • Respiratory swabs and urine pellets: freeze or refrigerate specimens promptly after collection. 
  • For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
​Shipping Instructions
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.  

Ship specimens and a hard copy of the completed submittal forms to: 
​CDPH VRDL  
ATTN: Specimen Receiving 
850 Marina Bay Parkway  
Richmond, CA 94804  
Phone: 510-307-8585 
Turnaround Time
10 Business Days
Limitations
​Negative results do not preclude the possibility of infection.
This test may not be used for diagnosis.
​Additional Information
Specimens should be collected as close to symptom onset as possible. 
​VRDL Points of Contact
Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov​​​
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