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Hepatitis A Virus Identification - Genotyping​

Info​rmation CategoryInformation Detail
Supplemental Information Requested

Specimens must have tested positive for Hepatitis A Virus IgM at an outside laboratory

Requested: disease onset date

Submittal FormSpecimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form​ (PDF)​ or a form generated in the VRDL Lab Web Portal.​
MethodologySequence based genotyping (LDT) (Surveillance Use Only)
Reflex TestingNone
Acceptable Specimen Type(s) and Collection MethodHuman specimens:
  • Serum or whole blood: red top or red/gray top serum separator tubes
  • ​​Plasma:  Whole blood with potassium EDTA
  • ​​Hepatitis virus RNA-positive nucleic acid extract
Minimum Volume Requested
  • ​​​Serum or Plasma: 0.5 mL
  • Whole blood in serum separator tubes: 2 mL
  • ​Whole blood in EDTA anticoagulant tubes: 2 mL
  • Nucleic acid extract: 30 uL
Transport Medium (if using)Not Applicable
Specimen LabelingEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions
  • Serum and Plasma: freeze or refrigerate specimens after collection.
  • Whole blood: refrigerate specimens promptly after collection. Do not freeze whole blood.
  • Nucleic acid extract: Freeze promptly

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

Shipping Instructions

Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.

Ship specimens and a hard copy of the completed submittal forms to: 

ATTN: Specimen Receiving
850 Marina Bay Parkway
Richmond, CA 94804
Phone: 510-307-8585

Turnaround Time20 Business days
LimitationsSpecimens with limiting amounts of viral nucleic acid may not be able to be sequenced
Additional InformationSpecimens should be collected as close to onset as possible.
VRDL Points of Contact
Medical and Epidemiology Liaisons: (510) 307-8585 or​​​

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