Supplemental Information or Form
LAB 444, EXAMINATION FOR MYCOBACTERIA TUBERCULOSIS DRUG
Performed on Specimens From
M. tb complex mixed with
non-TB mycobacteria or other bacteria.
suspected of having drug-resistant tuberculosis, patients who are especially
susceptible to treatment failure, cultures of
Acceptable Sample/Specimen Type for Testing
or culture - At least 0.5 ml of a concentrated sample which is acid-fast smear
positive. Smear negative samples need approval for submission
Minimum Volume Required
ml for sediment or broth, more if preferred
Storage & Preservation of Sample/Specimen Prior to Shipping
sediment, refrigerate. If culture, incubate at 35-37°C.
name, accession number
Shipping Instructions which Include Specimen Handling Requirements
cultures of M. tb complex as infectious substances. Sediments may be
shipped as diagnostic specimens with a cold pack.
method used to identify mutations associated with drug resistance is
pyrosequencing (PSQ). The molecular targets in the first line panel for detecting
isoniazid resistance are katG, inhA and ahpC
promoters and fabG1; that for rifampin is rpoB. The molecular
targets in the second line panel for detecting resistance to quinolones is
gyrA and that for kanamycin, amikacin capreomycin and kanamycin is
second line panel is tested when any mutation is detected in the first panel, or
more information on pyrosequencing, please contact us.
1 to 3
Interferences & Limitations
substances in the specimen may prevent successful DNA amplification and
MDL Points of Contact
Mycobacterial, Mycotic and Parasitic Diseases Section
TB DST Unit (510) 412-3929 or (510) 412-3949