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microbial diseases laboratory

Test Order Streptococcus pneumoniae Identification, Confirmation and Serotyping

Pre-Approval Required

Yes, if serotyping is requested. No, if identification or confirmation only is needed

Supplemental Information or Form

1.    Pure, viable subculture

2.    Completed submittal form with 2 patient identifiers (e.g. name and date of birth), collection date and source, a brief but complete patient history, cultural history and submitting laboratory’s findings.

3.    Documentation that indicates the role of this isolate in clinical disease

4.  LAB446 (11/07) (MDL-N-11)

5.  Vaccine history is required for serotyping 

Performed on Specimens From


Acceptable Sample/Specimen Type for Testing

Confirmed or suspected Streptococcus pneumoniae isolate

Minimum Volume Required


Storage & Preservation of Sample/Specimen Prior to Shipping



Tubed agar medium with a secure screw cap, labeled with patient’s name

Sample/Specimen Labeling


Shipping Instructions which Include Specimen Handling Requirements

1.    Grow isolate under appropriate conditions prior to shipping.

2.    Ship at ambient temperature using appropriate DOT/IATA approved shipping procedures.

Test Methodology

As needed - Biochemical testing, Gene Sequencing by PCR, and/ or MALDI-TOF mass spectrometry. Please note that serotyping will only be performed on isolates from sterile body sites, such as blood or cerebrospinal fluid, and with the approval of Dr. FengFeng Xu.

Turnaround Time

4 weeks

Interferences & Limitations

Mixed or nonviable culture

Additional Information

Please contact the MDL Vaccine Preventable Pathogens Unit for special consideration if any of the above criteria cannot be met.

MDL Points of Contact

Vaccine Preventable Pathogens Unit (510) 412-3700

Inger Vilcins (510) 412-3738

Monday-Friday 8 AM to 5 PM PT

Emails: or

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