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microbial diseases laboratory

Test Order Mycobacterium tuberculosis Complex Identification by Pyrosequencing Assay

Pre-Approval Required

None

Supplemental Information or Form

Form LAB 444 EXAMINATION FOR MYCOBACTERIA TUBERCULOSIS DRUG SUSCEPTIBILITY 

Performed on Specimens From

Sputum, or other sources

Acceptable Sample/Specimen Type for Testing

Culture or processed sediments

Minimum Volume Required

0.5ml for sediment or broth, more if preferred 

Storage & Preservation of Sample/Specimen Prior to Shipping

Incubated at 35-37°C (or refrigerated)

Transport

Tubed medium with screw cap tightened and taped

Sample/Specimen Labeling

Patient name, accession number

Shipping Instructions which Include Specimen Handling Requirements

Ship cultures of M. tb complex as infectious substances.

Test Methodology

  • The methodologies used are pyrosequencing (PSQ)

  • A PSQ assay targeting IS6110 is used for identification of Mycobacterium tuberculosis complex.

  • A PSQ assay targeting pncA is used for identification of Mycobacterium bovis. This assay does not differentiate between M. bovis and M. bovis BCG.

Turnaround Time

Average 1-3 days

Interferences & Limitations

Bacterial and/or fungal contaminants can interfere with the identification process.

Additional Information

Identification of Mycobacterium bovis is encouraged because the epidemiology is different from that of Mycobacterium tuberculosis.

MDL Points of Contact

Mycobacterial, Mycotic and Parasitic Diseases Section
TB DST Unit (510) 412-3929 or (510) 412-3949
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