Yes. Consult with Mycobacterial & Parasitic Diseases Reference Unit before submitting specimens. Specimens sent without prior approval may not be tested.
Supplemental Information or Form
Form LAB 402
Performed on Specimens From
Acceptable Sample/Specimen Type for Testing
Respiratory specimens (sputum, bronchial alveolar lavage, etc.), tissue or body fluid. Swabs are suboptimal for culture and AFB Smear.
Minimum Volume Required
5 mL recommended; 1 mL minimum
Storage & Preservation of Sample/Specimen Prior to Shipping
Store specimen at 2°C to 8°C; specimens should be submitted as soon as possible after collection.
Ship with cold packs. Do not freeze.
Testing subject to CLIA regulations require two patient identifiers on the specimen container and the test requisition. Patient’s name and collection date and time must be on specimen containers for samples processed for mycobacterial isolation and AFB smear. Additional acceptable identifiers include submitter’s accession number, patient ID, and date of birth.
Shipping Instructions which Include Specimen Handling Requirements
Ship as diagnostic specimen with cold packs. Do not freeze.
Digestion and decontamination, inoculation onto liquid and solid culture media, acid-fast microscopy using fluorescent auramine-rhodamine stain, phenotypic and molecular (MALDI-TOF and Sequencing) on AFB-positive cultures.
6 weeks for AFB negative cultures; up to 3 weeks if culture is positive for Mycobacterium tuberculosis complex and 4 weeks for mycobacteria other than M. tuberculosis complex. A preliminary report will be issued if culture becomes positive and AFB are observed.
Interferences & Limitations
The overgrowth of bacterial and/or fungal contaminants in positive cultures can prevent the isolation and identification of the acid-fast bacilli.
If M. tuberculosis complex is isolated from a new patient, drug susceptibility testing will be performed using culture and molecular methods (Pyrosequencing).
MDL Points of Contact
Mycobacterial & Parasitic Diseases Reference Unit