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microbial diseases laboratory branch

Tesā€‹ā€‹tā€‹ā€‹ Name 

Cyclospora ā€‹ā€‹Targeted Amplicon Deep Sequencing 

ā€‹

ā€‹Test ā€‹ā€‹ā€‹ā€‹ā€‹Mā€‹ā€‹ā€‹eā€‹thā€‹oā€‹ā€‹ā€‹ā€‹dā€‹ā€‹ā€‹ologā€‹yā€‹
 ā€‹ā€‹Next Generation Sequencingā€‹

ā€‹Reflex Testing
Criteria
ā€‹
ā€‹None
ā€‹Pre-Aā€‹pproval Requiredā€‹ā€‹
ā€‹
ā€‹Yes.ā€‹ Please contact MDL at MDL.Submissioā€‹ns@CDPHā€‹.ca.gov ā€‹
ā€‹Suppā€‹lemental Information and Required Form(s)ā€‹
ā€‹
ā€‹ā€‹Test Request Form for Cyclospora Targeted Amplicon Deep Sequencing (TADS). The submission form will be shared once shipment is approved.ā€‹ā€‹
ā€‹Requirā€‹ed Specimen Type(s)ā€‹
ā€‹ā€‹Stool specimen submitted either in a Cary Blair or at least 3 aliquots in 2 mL sterile microcentrifuge tubes. Please secure top of container/ tubes with parafilm to avoid leakage during transport. 

ā€‹Minimum Volume Required
ā€‹
If submitted in microcentrifuge tube, 2 mL.

ā€‹Specimen Stabilityā€‹

4ā€‹Ā°C up to 15 days 

ā€‹ā€‹Rejection Criteriaā€‹

ā€‹ā€‹Specimens collected in Formalin will be rejected

Storage/Transportā€‹
Conditions
ā€‹
ā€‹
ā€‹4ā€‹Ā°C,  ā€‹-20Ā°C or other (please indicate on Test Request Form)

ā€‹Transport Medium,ā€‹
if applicable
ā€‹

ā€‹Cary-Blair modified media or VTM (Viral Transport Media)ā€‹ā€‹

ā€‹ā€‹Speā€‹cimen Labelingā€‹
ā€‹ā€‹Containing patient identifiers: name, DOB, and collection date

ā€‹Shipping Instructions and Specimen Handling Requirementsā€‹
ā€‹ā€‹ā€‹ā€‹ā€‹Specimen(s) should be shipped according to International Air Transport Association (IATA) and International Civil Aviation Organization (ICAO) regulations for biological specimens.ā€‹ā€‹

Ship to:

California Departā€‹ment of Public Healthā€‹
Microbial Diseases Laboratory ā€‹
ATTN:  ā€‹ā€‹Molecular Characterization Unitā€‹ā€‹
850 Marina Bay Parkway, Specimen Receiving Room B106
Richmond, CA  94804
ā€‹Specā€‹ā€‹imen Collection Instructions, if applicable

ā€‹Not Applicable
ā€‹Turnaround Time

ā€‹ā€‹Not Applicableā€‹
ā€‹ā€‹Interferences & Limitationsā€‹
Noneā€‹
ā€‹Reference Rangeā€‹

ā€‹ā€‹Not Applicableā€‹ā€‹ā€‹ā€‹
ā€‹Additional Informationā€‹
ā€‹None
ā€‹ā€‹MDL Point of Contactā€‹

ā€‹MDL - Molecular Characterization Unit 
Phone: (510) 412-3940, Mā€“F PST.ā€‹
Email MDL.Submissions@CDPH.ca.gov 
ā€‹
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