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microbial diseases laboratory

Test Order - Carbapenemase Gene Detection

Pre-Approval Required

Isolates must be confirmed as Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae,
Enterobacter species or Pseudomonas aeruginosa.
At this time, acceptance of requests for testing of other Enterobacteriaceae organisms is on a case by case basis after consultation with HAI program at HAIprogram@cdph.ca.gov.
Isolates of Enterobacteriaceae, other than those listed above, that are submitted without prior consultation will not be tested.

Supplemental Information or Form

AST-R Submittal form 08182017

MDL Expanded Carbapenemase Testing Frequently Asked Questions (FAQ)

Performed on Specimens From

Human source

Acceptable Sample/Specimen Type for Testing

Pure culture isolate.

Isolates must be resistant to at least one carbapenem, i.e., imipenem, ertapenem, doripenem, or meropenem.

Isolates must be identified to at least the genus level by the submitter.

Mixed cultures received in MDL will not be tested.

Minimum Volume Required

None

Storage & Preservation of Sample/Specimen Prior to Shipping

Store at ambient temperature

Transport

Ship cultures on nonselective nutrient or similar agar in tubes with leak-proof screw cap closures.

Sample/Specimen Labeling

Testing subject to CLIA regulations require two patient identifiers on the specimen container and the test requisition

Shipping Instructions which Include Specimen Handling Requirements

Ship at ambient temperature.

Test Methodology

PCR

Turnaround Time

7-10 days

Interferences & Limitations

None

Additional Information

None

MDL Points of Contact

MDL - Foodborne & Waterborne Diseases Section (510) 412-3796

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