Pre-Approval
|
Not Applicable
|
Supplemental Information Requested
|
Requested: Travel history, rash onset date, symptoms, measles vaccination history, contacts to known case(s)
|
Submittal Form
|
Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form (PDF) or a form generated in the VRDL Lab Web Portal.
|
Methodology
|
Sequence-based genotyping (LDT) (Surveillance use only)
|
Reflex Testing
|
May be reflexed to whole genome sequencing
|
Acceptable Specimen Type(s) and Collection Method
|
Human specimens:
- Respiratory specimens: Throat (preferred) and/or NP swabs in sterile collection tube with liquid viral transport media
- Urine: Processed urine preferred
- Measles-positive nucleic acid extracts
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft.
To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8°C. Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
|
Minimum Volume Requested
|
- Respiratory swab: 2–3 mL of Universal or Viral Transport Medium
- Urine, unprocessed: 10-50 mL
- Urine, processed: Pellet resuspended in 2–3 mL of Universal or Viral Transport Medium
- Nucleic acid extracts: 25-50 µL
|
Transport Medium (if using)
|
For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium
|
Specimen Labeling
|
Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
|
Storage & Preservation of Specimen / Shipping Conditions
|
- Respiratory swabs and urine pellets: freeze or refrigerate specimens promptly after collection.
- For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
|
Shipping Instructions
|
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.
Ship specimens and a hard copy of the completed submittal forms to:
CDPH VRDL
ATTN:
Specimen Receiving
850 Marina Bay Parkway
Richmond, CA 94804
Phone: 510-307-8585
|
Turnaround Time
|
10 Business Days
|
Limitations
|
Negative results do not preclude the possibility of infection.
This test may not be used for diagnosis.
|
Additional Information
|
Specimens should be collected as close to symptom onset as possible.
|
VRDL Points of Contact
|
Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov
|