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Viral and Rickettsial Disease Laboratory​

Test Order 
Anaplasma phagocytophilum (HGE) Detection - PCR​

Information Category Information Detail
Pre-Approval None
Supplemental Information Requested Requested: Travel history, symptoms, exposures (including animals, arthropods, etc), disease onset, clinical summary (e.g. antibiotic therapy and dates) 
Submittal Form Specimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form (PDF) or a form generated in the VRDL Lab Web Portal.
Methodology (Commercial Test Name or Laboratory Developed Test (LDT)) Real-time PCR (LDT)(Surveillance use only)
Reflex Testing None
Acceptable Specimen Type(s) and Collection Method

Human specimens: 
  • Serum:  
    • ​Plain Red-Top Tubes: Serum collected in plain, red-top tubes must be separated and transferred to sterile, screw-cap tubes for storage and transport.
    • Serum Separator Tubes (SST): SST may be transported after centrifugation.
  • Plasma: Plasma must be separated from EDTA (lavender top) whole blood within 2 hours of collection and transferred to sterile, screw-cap tubes for storage and transport 
  • Whole Blood: EDTA (lavender top) tubes 
Minimum Volume Requested​
  • Serum: 1 mL
  • Whole Blood: 5 mL
  • Plasma: 1 mL 
Transport Medium (if using) Not Applicable
Specimen Labeling Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patie​nt full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions
  • Separated Serum: Refrigerate or freeze separated serum promptly after collection.
  • Serum Separator Tubes: Refrigerate, do not freeze.
  • Plasma: Freeze specimens promptly after collection. Plasma must be separated from EDTA whole blood and frozen within 2 hours of collection. 
  • Whole Blood: Refrigerate, do not freeze.

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

Shipping Instructions
​Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.

Ship specimens and a hard copy of the completed submittal forms to: 

​CDPH VRDL

ATTN: Specimen Receiving

850 Marina Bay Parkway Richmond, CA 94804

Phone: 510-307-8585

​Turnaround Time
​10 Business Days
​​Limitations

​Negative result does not preclude the possibility of infection
​Additional Information
​Specimens should be collected as close to illness onset as possible.
​VRDL Points of Contact
Medical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov

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