​Test Order – STEC Isolation from Enrichment Broth

Pre-Approval Required

None

Supplemental Information or Form

The submitting laboratory is encouraged to isolate STEC and submit suspect isolates along with broth.

 ​Use MDL Lab Web Portal for ordering a test. Select Test Requisition “Feces for Bacterial Culture – Form 414” for submission of the broth and “Bacterial Culture for Identification – Form 446”​  for submission of suspect STEC isolates.

Performed on Specimens From

Human source

Acceptable Sample/Specimen Type for Testing

GN or MAC Broth positive by Shiga toxin testing

Minimum volume required: 5 ml (broth)

Minimum Volume Required

5 ml (broth)

Storage & Preservation of Sample/Specimen Prior to Shipping

Store specimen at 4°C

Transport

For isolates accompanying broth, ship cultures on nonselective nutrient or similar agar in tubes with leak-proof screw cap closures.

Sample/Specimen Labeling

Testing subject to CLIA regulations require two patient identifiers on the specimen container and the test requisition.

Shipping Instructions which Include Specimen Handling Requirements

 ​Transport all clinical specimens refrigerated using frozen gel packs. Do not use wet or dry ice.

​Test Methodology

Isolation, Phenotypic Identification, PCR

Turnaround Time

15 to 45 days

Interferences & Limitations

None

Additional Information

None

MDL Points of Contact

MDL - Foodborne & Waterborne Diseases Section (510) 412-3796 

Monday-Friday 8 AM to 5 PM PT

Email: MDL.Submissions@CDPH.ca.gov