Test Order - Bordetella Pertussis Real-Time PCR
Pre-Approval Required
None
Supplemental Information or Form
1.
Nasopharyngeal swab aspirate from a patient with a high clinical suspicion of
whooping cough that has been exposed to a confirmed case or is part of a
suspected outbreak.
2. A
completed
submittal form with 2 patient identifiers (e.g. name and date of birth). Please
include the date of collection and information on the patient's suspected
disease, clinical findings, antibiotics administered, and culture
results.
3.
VPP-Form03.Pertussis PCR Test Request
Performed on Specimens From
None
Acceptable Sample/Specimen Type for Testing
Nasopharyngeal
swabs are the preferred specimens. This MUST be a Dacron swab. Place swab into
Regan-Lowe transport medium immediately after collection. Dry swabs are also
acceptable for PCR testing.
Nasopharyngeal aspirates
Minimum Volume Required
None
Storage & Preservation of Sample/Specimen Prior to Shipping
None
Transport
None
Sample/Specimen Labeling
None
Shipping Instructions which Include Specimen Handling Requirements
Ship
at ambient temperature using appropriate DOT/IATA approved shipping
procedures. Swabs should be shipped immediately at ambient temperature, or
incubated for 48 hours at 35 Ā°C
prior to transport if immediate shipment is not possible.
Test Methodology
Real-time PCR
Turnaround Time
72 hours
Interferences & Limitations
Culture
positive specimens or those collected from nonsterile body sites are not
apprpriate for this testing
Additional Information
Please
contact the MDL Vaccine
Preventable Pathogens Unit for special consideration if any of the above
criteria cannot be met.
MDL Points of Contact
Vaccine
Preventable Pathogens Unit (510) 412-3700
Monday-Friday
8 AM to 5 PM PT
Emails:
Jennifer.kyle@cdph.ca.gov; Nirmala.dhungana@cdph.ca.gov; laura.smoot@cdph.ca.gov