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​​microbial diseases laboratory branch

Tes​​t​​ Name 

Vibrio Fecal Culture (positive Culture-Independent Diagnostic Test (CIDT) only)​​

  

Elegible Submitters​​

​​Test is available to all pre-approved submitters within the state of California. Submissions from clinical laboratories should route through the corresponding county public health laboratories (PHL). 

T​est M​​​e​th​​o​​d​​​olog​y​​
​Phenotypic or genotypic Identification, serotyping, biotyping, virulence testing for cholera toxin gene​.
​​
Reflex Testing
Criteria

​Recovered bacterial isolates may be routed for molecular characterization using Whole Genome Sequencing (WGS) for public health surveillance and epidemiologic investigations. Clinical reports are not issued for surveillance or epidemiologic testing.

P​​​​re-​A​pproval Required​

None


Supp​lem​ental Information and Required Form(s)​

​Fecal specimen must be positive by a CIDT for Vibrio species.

​Use MDL Lab Web Portal for ordering the test, select Test Requisition for ​​“Feces for Bacterial Culture – 414​” 

​Test requested: 

Vibrio cholerae fecal culture 

or

Vibrio species (cholerae, parahaemolyticus, vulnificus) fecal culture.

For submitters who do not have access to the MDL LWP; please contact MDL.Submissions@CDPH.ca.gov for additional information.​​​


Required Specimen Type(s)


​Human source
Feces in transport medium (preferably Cary-Blair)​.
Mini​​​mum​​ Volume Required ​​Fecal specimen 0.5-1 gram-mL or fecal swab.

Specimen Stability

 

​Fecal specimens in transport medium from collection to receipt ≤14 days at 2-8​°​​C​.

​Rejection​ Criteria

 

  • ​Submissions with insufficient or conflicting labeling
  • Broken or leaking samples
  • Specimens sent without prior approval
  • Fecal specimens received <2°​​C
  • Fecal specimens received >8°​​C
  • Fecal specimen in an enrichment broth
  • No fecal material observed in transport medium
  • Frozen specimens
  • ​​Specimen ≥15 calendar days from date of collection to date received
  • Specimen in Buffered Glycerol Saline (BGS) transport medium

Storage/Transport Conditions ​​​

​​​Store specimen in transport medium refrigerated at 2-8°C 
Transport Medium,​
if applicable

​Cary-Blair (Preferred) 
Buffered Glycerol Saline is unsatisfactory for Vibrio species 
Specim​en Labeling ​​​Testing subject to CLIA regulations. Minimum label requirement on the specimen includes patient name and one other identifier (e.g. preferably submitter sample ID, or patient medical record number, or patient date of birth) on the specimen container and the test requisition.

Shipping Instructions and Specimen Handling Requirements

 

 

 

 

  • ​​Transport all clinical specimens refrigerated at 2-8°​​​C using frozen gel packs
  • Frozen gel packs should not be in direct contact with specimen 
  • Do not use wet or dry ice
  • Do not freeze specimen
  • The shipper is responsible for making sure that all samples and specimens are packaged and shipped according to current federal and state packaging and shipping regulations for Category A infectious substance and/or Category B diagnostic samples

Ship to:

California Depart​ment of Public Health​
Microbial Diseases Laboratory ​
ATTN: Food and Water Diseases Section​
850 Marina Bay Parkway, Specimen Receiving Room B106
Richmond, CA  94804​

Specimen Collection Instructions, if applicable
Not applicable
Turnaround Time 14-30 days

Interfer​ences & Limitations ​​Buffered Glycerol Saline is inhibitory to Vibrio species  

Reference Range Not applicable

Additional Information None


MDL ​Point of Contact ​MDL - Foodborne & Waterborne Diseases Section (510) 412-3796

Monday–Friday 8 AM to 5 PM PT

Email: MDL.Submissions@CDPH.ca.gov

​​​

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