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Elegible Submitters |
Test is available to all pre-approved submitters within the state of California. Submissions from clinical laboratories should route through the corresponding county public health laboratories (PHL).
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Test Methodology
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Phenotypic or genotypic Identification, serotyping, biotyping, virulence testing for cholera toxin gene.
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Reflex Testing Criteria |
Recovered bacterial isolates may be routed for molecular characterization using Whole Genome Sequencing (WGS) for public health surveillance and epidemiologic investigations. Clinical reports are not issued for surveillance or epidemiologic testing.
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Pre-Approval Required |
None
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Supplemental Information and Required Form(s) |
Fecal specimen must be positive by a CIDT for
Vibrio species.
Use MDL Lab Web Portal for ordering the test, select Test Requisition for “Feces for Bacterial Culture – 414”
Test requested:
Vibrio cholerae fecal culture
or
Vibrio species (cholerae, parahaemolyticus, vulnificus) fecal culture.
For submitters who do not have access to the MDL LWP; please contact
MDL.Submissions@CDPH.ca.gov for additional information.
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Required Specimen Type(s)
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Human source
Feces in transport medium (preferably Cary-Blair).
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Minimum Volume Required |
Fecal specimen 0.5-1 gram-mL or fecal swab.
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Specimen Stability
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Fecal specimens in transport medium from collection to receipt ≤14 days at 2-8°C.
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Rejection Criteria
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- Submissions with insufficient or conflicting labeling
- Broken or leaking samples
- Specimens sent without prior approval
- Fecal specimens received <2°C
- Fecal specimens received >8°C
- Fecal specimen in an enrichment broth
- No fecal material observed in transport medium
- Frozen specimens
- Specimen ≥15 calendar days from date of collection to date received
- Specimen in Buffered Glycerol Saline (BGS) transport medium
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Storage/Transport Conditions
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Store specimen in transport medium refrigerated at 2-8°C
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Transport Medium, if applicable
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Cary-Blair (Preferred) Buffered Glycerol Saline is unsatisfactory for Vibrio species
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Specimen Labeling |
Testing subject to CLIA regulations. Minimum label requirement on the specimen includes patient name and one other identifier (e.g. preferably submitter sample ID, or patient medical record number, or patient date of birth) on the specimen container and the test requisition.
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Shipping Instructions and Specimen Handling Requirements
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- Transport all clinical specimens refrigerated at 2-8°C using frozen gel packs
- Frozen gel packs should not be in direct contact with specimen
- Do not use wet or dry ice
- Do not freeze specimen
- The shipper is responsible for making sure that all samples and specimens are packaged and shipped according to current federal and state packaging and shipping regulations for Category A infectious substance and/or Category B diagnostic samples
Ship to: California Department of Public Health Microbial Diseases Laboratory ATTN: Food and Water Diseases Section 850 Marina Bay Parkway, Specimen Receiving Room B106 Richmond, CA 94804
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Specimen Collection Instructions, if applicable
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Not applicable |
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Turnaround Time |
14-30 days
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Interferences & Limitations |
Buffered Glycerol Saline is inhibitory to Vibrio species
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Reference Range |
Not applicable
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Additional Information |
None
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MDL Point of Contact |
MDL - Foodborne & Waterborne Diseases Section (510) 412-3796
Monday–Friday 8 AM to 5 PM PT
Email: MDL.Submissions@CDPH.ca.gov
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