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Test Order for Hepatitis A Virus Identification - Genotyping

VIRAL AND RICKETTSIAL DISEASE LABORATORY

Test Order

Hepatitis A Virus Identification - Genotyping

Information Category	Information Detail
Pre-Approval	None
Supplemental Information Requested	Specimens must have tested positive for Hepatitis A Virus IgM at an outside laboratory Requested: disease onset date
Submittal Form	VRDL General Purpose Specimen Submittal Form
Methodology	Sequence based genotyping (LDT) (Surveillance Use Only)
Reflex Testing	None
Acceptable Specimen Type(s) and Collection Method	Human specimens: Serum or whole blood: red top or red/gray top serum separator tubes Plasma: Whole blood with potassium EDTA Hepatitis virus RNA-positive nucleic acid extract
Minimum Volume Requested	Serum or Plasma: 0.5 ml Whole blood in serum separator tubes: 2 ml Whole blood in EDTA anticoagulant tubes: 2 ml Nucleic acid extract: 30 ul
Transport Medium (if using)	Not Applicable
Specimen Labeling	Each specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions	Serum and Plasma: freeze or refrigerate specimens after collection. Whole blood: refrigerate specimens promptly after collection. Do not freeze whole blood. Nucleic acid extract: Freeze promptly Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
Shipping Instructions	Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment. Ship specimens and a hard copy of the completed submittal forms to: CDPH VRDL ATTN: Specimen Receiving 850 Marina Bay Parkway Richmond, CA 94804 Phone: 510-307-8586
Turnaround Time	20 Business days
Limitations	Specimens with limiting amounts of viral nucleic acid may not be able to be sequenced
Additional Information	Specimens should be collected as close to onset as possible.
VRDL Points of Contact	Medical and Epidemiology Liaisons: (510) 307-8587

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