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VIRAL AND RICKETTSIAL DISEASE LABORATORY

 Test Order​​​​​
Dengue Virus - Neutralizing Antibody Test

Information Cate​​gory​​​​Information Detail
Pre-ApprovalNone
Supplemental Information RequestedRequested: Travel history, illness onset date, IgM test result for requested virus
Submittal FormSpecimens must be accompanied with a hard copy of the completed VRDL General Purpose Specimen Submittal Form​ or a form generated in the VRDL Lab Web Portal.
Methodology (Commercial Test Name or Laboratory Developed Test (LDT))Plaque Reduction Neutralization Test or “PRNT" (LDT) (Diagnostic)
Reflex TestingNone
Acceptable Specimen Type(s) and Collection Method

Human specimens:

  • Serum: serum tubes (red top) or serum separator tubes (red/gray top)
  • CSF: sterile collection tubes
Minimum Volume RequiredSerum: 1 mL
Whole blood in serum separator tubes: 5-8 mL
CSF: 1 mL
Transport Medium (if using)Not Applicable
Specimen LabelingEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
Storage & Preservation of Specimen / Shipping Conditions

Serum: freeze or refrigerate specimens promptly after collection.

Whole blood: refrigerate specimens promptly after collection. Do not freeze whole blood.

CSF: freeze or refrigerate specimens promptly after collection.

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

Shipping Instructions

 

Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.

Ship specimens and a hard copy of the completed submittal forms to:    

CDPH VRDL

ATTN: Specimen Receiving

850 Marina Bay Parkway

Richmond, CA 94804

Phone: 510-307-8585

Turnaround Time16 Business Days
Limitations

Does not distinguish between dengue serotypes.

Flavivirus antibodies are often cross-reactive.

PRNT is performed with paired viruses that are closely related and geographically co-circulating. Results for both viruses are reported. Traditionally, where neutralizing antibodies are detected for both viruses, a four-fold difference in titer is presumed to indicate the identity of the exposure; however, due to the possibility of an anamnestic antibody response, single specimens are screened at low dilutions and identity is only reported when neutralization is detected for only one virus. Dual reactivity is reported as evidence of undifferentiated flavivirus exposure.

Laboratory results with clinical and environmental correlation must be used together with current case definitions to confirm or rule out cases.

Additional InformationA convalescent specimen is recommended to help determine recency of infection. When submitted separately, be sure to identify the previously submitted specimen by the VRDL accession number so that the acute and convalescent specimens may be linked, tested to endpoint and interpreted as a pair.
VRDL Points of ContactMedical and Epidemiology Liaisons: (510) 307-8585 or VRDL.submittal@cdph.ca.gov 

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