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microbial diseases laboratory

Test Order STEC Isolation for Enrichment Broth

Pre-Approval Required

None

Supplemental Information or Form

The submitting laboratory is encouraged to isolate STEC and submit suspect isolates along with broth.  Bacterial Culture for Identification Form “LAB 446” is required for submission of samples.

Performed on Specimens From

Human source

Acceptable Sample/Specimen Type for Testing

GN or MAC Broth positive by Shiga toxin testing

Minimum volume required: 5 ml (broth)

Minimum Volume Required

5 ml (broth)

Storage & Preservation of Sample/Specimen Prior to Shipping

Store specimen at 4°C.

Transport

For isolates accompanying broth, ship cultures on nonselective nutrient or similar agar in tubes with leak-proof screw cap closures.

Sample/Specimen Labeling

Testing subject to CLIA regulations require two patient identifiers on the specimen container and the test requisition.

Shipping Instructions which Include Specimen Handling Requirements

Ship at ambient temperature.

Test Methodology

Isolation, Phenotypic Identification, PCR, Vero Cell Assay

Turnaround Time

15 to 45 days

Interferences & Limitations

None

Additional Information

None

MDL Points of Contact

MDL - Foodborne & Waterborne Diseases Section (510) 412-3796, Monday-Friday 8 AM to 5 PM PT

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