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Test Order
Mpox Virus Detection - PCR

​Information Category
​Information Detail

Not Applicable

​Supplemental Information Required
Not Applicable
​Submittal Forms

VRDL General Purpose Specimen Submittal Form

(Mpox group submittal form available on request from


Real-time PCR (LDT) (Diagnostic)

Reflex Testing
  • May be reflexed to Non-variola Orthopox Virus
  • May be reflexed to Varicella Zoster and/or Herpes Simplex Virus
  • May be referred to CDC for additional testing
​Acceptable Specimen Type(s) and Collection Method
Human Specimens:
  • Lesion swabs, in Viral Transport Medium (preferred) or dry
  • Dry lesion scabs or crusts
​Minimum Volume Required
Submitters should sample at least  two (2) Mpox lesions per patient (maximum 4 lesions). Each lesion should be sampled with 2 separate swabs to accommodate confirmatory testing, if needed. 
​Transport Medium (if using)
For lesion swabs only: 1–3 ml of Viral Transport Medium
​Specimen Labeling
​Each specimen tube must be labeled with at least two unique patient identifiers; e.g., patient full name and date of birth.
​Storage & Preservation of Specimen / Shipping Conditions

Lesion swabs: freeze or refrigerate specimens promptly after collection. Freezing is strongly recommended.

Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.

​Shipping Instructions
Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance – Category B (UN 3373) shipment.  

Ship specimens and a hard copy of the completed submittal forms to:
     ATTN: Specimen Receiving
     850 Marina Bay Parkway
     RIchmond, CA 94804
     Phone: 510-307-8585
Turnaround Time
5 business days
Interferences & ​Limitations
​Negative results do not preclude the possibility of infection.
​Additional Information
​This assay can distinguish between Mpox Clades I and II viruses. 

​VRDL Points of Contact
Medical and Epidemiology Liaisons: (510) 307-8585 or​ 
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