Pre-Approval
| Not Applicable
|
āSupplemental Information Required or Requested
| Requested: Measles vaccination history, travel history, rash onset date, symptoms, if contacts to known case(s)
|
āSubmittal Form
| VRDL General Purpose Specimen Submittal Form
|
Methodology
| Real-time RT-PCR (LDT) (Diagnostic)
|
Reflex Testing
| Genotyping
|
āAcceptable Specimen Type(s) and Collection Method
| Human specimens:
- Respiratory specimens: Throat and/or NP swab in sterile collection tube with liquid Viral Transport Media or Universal Transport Medium
- Urine: Processed urine preferred
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft.
To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8Ā°C. Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
|
āMinimum Volume Required
| Respiratory swab: 2-3 mL of Universal or Viral Transport Medium
Urine, unprocessed: 10-50 mL
Urine, processed: Pellet resuspended in 2-3 mL of Universal or Viral Transport Medium
|
āTransport Medium (if using)
| For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium
|
āSpecimen Labeling
| āEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
|
āStorage & Preservation of Specimen / Shipping Conditions
| For respiratory swabs or urine pellet, freeze or refrigerate specimens promptly after collection.
For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice.
|
āShipping Instructions
| Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā Category B (UN 3373) shipment.
Ship specimens and a hard copy of the completed submittal forms to:
CDPH VRDL ATTN: Specimen Receiving 850 Marina Bay Parkway Richmond, CA 94804 Phone: 510-307-8585
|
Turnaround Time
| 3 Business Days
|
Limitations
|
|
āAdditional Information
| āThis test is intended for persons who received a measles-containing vaccine within the preceding 28 days and for whom there is concern of wildtype measles infection. Specimens should be collected as close to rash onset as possible and preferably within 30 days after receipt of a measles-containing vaccine.
|
āVRDL Points of Contact
| Medical and Epidemiology Liaisons: (510) 307-8585
|