Pre-Approval
| Not Applicable
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āSupplemental Information Required or Requested
| Requested: Travel history, rash onset date, symptoms, measles vaccination history, contacts to known case(s)
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āSubmittal Form
| VRDL General Purpose Specimen Submittal Form
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Methodology
| Real-Time RT-PCR (LDT) (Diagnostic)
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Reflex Testing
| Genotyping
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āAcceptable Specimen Type(s) and Collection Method
| Human specimens: - Throat (preferred) and/or NP swab in sterile collection tube with liquid viral transport media
- Urine: Processed urine preferred
Respiratory swabs must be a Dacron or synthetic tip with aluminum or plastic shaft.
To process urine, centrifuge urine sample (10-50 mL) at 500-600 x g for 5-10 min at 2-8Ā°C. Discard supernatant and resuspend cell sediment in 2-3 mL of viral transport media.
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āMinimum Volume Required
| Respiratory swab: 2-3 mL of Universal or Viral Transport Medium Urine, unprocessed: 10-50 mL
Urine, processed: Pellet resuspended in 2-3 mL of Universal or Viral Transport Medium
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āTransport Medium (if using)
| For respiratory swabs only: 2-3 mL of Universal or Viral Transport Medium
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āSpecimen Labeling
| āEach specimen tube must be labeled with at least two unique patient identifiers, e.g., patient full name and date of birth.
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āStorage & Preservation of Specimen / Shipping Conditions
| Respiratory swabs and urine pellets: freeze or refrigerate specimens promptly after collection.
For unprocessed urine, refrigerate specimens promptly after collection. Do not freeze unprocessed urine.
Ship refrigerated specimens to VRDL on cold packs. Ship frozen specimens to VRDL on dry ice. |
āShipping Instructions
| Work with your local public health department to ensure samples are packaged according to instructions for Biological Substance ā Category B (UN 3373) shipment.
Ship specimens and a hard copy of the completed submittal forms to:
CDPH VRDL ATTN: Specimen Receiving 850 Marina Bay Parkway
Richmond, CA 94804 Phone: 510-307-8585
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Turnaround Time
| 2 Business Days
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Limitations
| āNegative results do not preclude the possibility of infection
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āAdditional Information
| Specimens should be collected as close to rash onset as possible and preferably within 14 days of rash onset.
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āVRDL Points of Contact
| Medical and Epidemiology Liaisons: (510) 307-8585
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