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 Test Order

Mycobacterium tuberculosis
Complex Identification to Species

Pre-Approval Required

None

Supplemental Information or Form

Form LAB 443 (8/01)

Performed on Specimens from

Sputum, tissue or body fluid

Acceptable Sample/Specimen Type for Testing

Pure culture

Minimum Volume Required

None

Storage & Preservation of Specimen Prior to Shipping

Incubated at 35-37°C (or refrigerated).

Transport Medium

Tubed medium with screw cap tightened and taped.

Specimen Labeling

Patient name, accession number

Shipping Instructions which Include Specimen Handling Requirements

Ship cultures of M. tb complex as infectious substances.

Test Methodology

Pyrosequencing (PSQ) or AccuProbe (AP) or High Performance Liquid Chromatography (HPLC) for M. tuberculosis complex.  Pyrazinamide (PZA) susceptibility testing using Mycobacteria Growth Indicator Tube (MGIT) for M. tb (susceptible).  PZA resistance could be M. bovis or mono-resistant TB.  A combination of growth characteristics and spacer oligo-nucleotide typing (spoligotyping)  separates M. tb, and M. bovis (includes BCG).  High Performance Liquid Chromatography (HPLC) definitively identifies  M. bovis BCG vaccine strain.

Turnaround Time

1 to 2 weeks

Interferences & Limitations

Bacterial and/or fungal contaminants can interfere with the identification process.

Additional Information

Identification of Mycobacterium bovis is encouraged because the epidemiology is different from that of Mycobacterium tuberculosis.

Mycobacteriology and Mycology Contact Number

(510) 412-3924

 

 
 
Last modified on: 10/8/2013 10:36 AM