Supplemental Information or Form
Form LAB 443 (8/01)
Performed on Specimens from
Sputum, tissue or body fluid
Acceptable Sample/Specimen Type for Testing
Minimum Volume Required
Storage & Preservation of Specimen Prior to Shipping
Incubated at 35-37°C (or refrigerated).
Tubed medium with screw cap tightened and taped.
Patient name, accession number
Shipping Instructions which Include Specimen Handling Requirements
Ship cultures of M. tb complex as infectious substances.
Pyrosequencing (PSQ) or AccuProbe (AP) or High Performance Liquid Chromatography (HPLC) for M. tuberculosis complex. Pyrazinamide (PZA) susceptibility testing using Mycobacteria Growth Indicator Tube (MGIT) for M. tb (susceptible). PZA resistance could be M. bovis or mono-resistant TB. A combination of growth characteristics and spacer oligo-nucleotide typing (spoligotyping) separates M. tb, and M. bovis (includes BCG). High Performance Liquid Chromatography (HPLC) definitively identifies M. bovis BCG vaccine strain.
1 to 2 weeks
Interferences & Limitations
Bacterial and/or fungal contaminants can interfere with the identification process.
Identification of Mycobacterium bovis is encouraged because the epidemiology is different from that of Mycobacterium tuberculosis.
Mycobacteriology and Mycology Contact Number
| Conditions of Use