Supplemental Information or Form
Form 402 (10/11) or 402(5/03)
Performed on Specimens from
California public health laboratories with prior approval.
Acceptable Sample/Specimen Type for Testing
Sputum, tissue or body fluid
Minimum Volume Required
5 ml recommended, one ml minimum
Storage & Preservation of Specimen Prior to Shipping
Patient’s name, accession number
Shipping Instructions which Include Specimen Handling Requirements
Ship as diagnostic specimen.
Digestion and decontamination, inoculation into MGIT broths and onto solid media, acid-fast microscopy using fluorescent auramine-rhodamine stain.
6 to 7 weeks for negative cultures, approx. 3 weeks if positive for M. tuberculosis (TB) complex and mycobacteria other than M. tuberculosis complex.
Interferences & Limitations
The overgrowth of bacterial and/or fungal contaminants in positive cultures can prevent the isolation and identification of the acid-fast bacilli seen in smears.
If M. tuberculosis complex is isolated from a new patient, drug susceptibility testing will be performed using culture or molecular methods.
MDL Points of Contact
Mycobacterial Diseases Section (510) 412-3924 or (510) 412-3781Emails: email@example.com or firstname.lastname@example.org
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