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Test Order

Mycobacterium TB Complex Identification and Pyrazinamide Susceptibility Testing

Pre-Approval Required


Supplemental Information or Form


Performed on Specimens from

Human patients

Acceptable Sample/ Specimen Type for Testing

Pure culture

Minimum Volume Required

1 ml liquid with visible bimass or solid medium culture less than 6 week old

Storage & Preservation of Specimen Prior to Shipping


Transport Medium


Specimen Labeling


Shipping Instructions which Include Specimen Handling Requirements


Test Methodology

TB complex identification may use high performance liquid chromatography to identify BCG vaccine strains, or a combination of growth characteristics and spacer oligonucleotide typing (spoligotyping) to identify M. bovis.  Pyrazinamide susceptibility testing is performed using Mycobacterial Growth Indicator Tube (MGIT) broth.

Turnaround Time


Interferences & Limitations


Additional Information

Cultures of M. bovis are resistant to pyrazinamide (PZA).  Members of the TB complex which are resistant only to PZA are very likely to be M. bovis.

MDL Points of Contact

(510) 412-3924


Last modified on: 10/8/2013 11:53 AM