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Test Order

Mycobacterium TB Complex Identification and Pyrazinamide Susceptibility Testing

Pre-Approval Required

None

Supplemental Information or Form

None

Performed on Specimens from

Human patients

Acceptable Sample/ Specimen Type for Testing

Pure culture

Minimum Volume Required

1 ml liquid with visible bimass or solid medium culture less than 6 week old

Storage & Preservation of Specimen Prior to Shipping

None

Transport Medium

None

Specimen Labeling

None

Shipping Instructions which Include Specimen Handling Requirements

None

Test Methodology

TB complex identification may use high performance liquid chromatography to identify BCG vaccine strains, or a combination of growth characteristics and spacer oligonucleotide typing (spoligotyping) to identify M. bovis.  Pyrazinamide susceptibility testing is performed using Mycobacterial Growth Indicator Tube (MGIT) broth.

Turnaround Time

None

Interferences & Limitations

None

Additional Information

Cultures of M. bovis are resistant to pyrazinamide (PZA).  Members of the TB complex which are resistant only to PZA are very likely to be M. bovis.

MDL Points of Contact

(510) 412-3924

 

 
 
Last modified on: 10/8/2013 11:53 AM