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Mycobacterium tuberculosis Complex: Genotyping

Pre-Approval Required


Supplemental Information or Form

Submittal form is online, or through Centers for Disease Control and Prevention (CDC) tuberculosis genotyping information and management system (TBGIMS).

Performed on Specimens from

Public health laboratories

Acceptable Sample/Specimen Type for Testing

Pure cultures on liquid or solid medium.  M.tb complex only.

Minimum Volume Required

Liquid medium: 1 ml with visible biomass.  Solid medium culture: visible growth after at least two weeks incubation.

Storage & Preservation of Specimen Prior to Shipping


Transport Medium


Specimen Labeling

Submitter lab number; patient name optional

Shipping Instructions which Include Specimen Handling Requirements

Ship as infectious substance.  Pre-submit Excel or TBGIMS submittal form.

Test Methodology

Spacer oligonucleotide typing (“spoligotyping”) and MIRU/VNTR (mycobacterial interspersed repetitive units/variable number of tandem repeats).  RFLP on request from tuberculosis control programs for strains that match by spoligotyping and MIRU/VNTR.

Turnaround Time

Spoligo/MIRU 2 to 3 weeks, RFLP an additional 4 weeks.  More rapid turnaround may be possible in exceptional circumstances.

Interferences & Limitations

Unable to obtain results if culture mixed with another mycobacteria or with bacterial and/or fungal contaminants.

Additional Information


Mycobacteriology and Mycology Contact Number

(510) 412-6237 or (510) 412-3949

Last modified on: 10/8/2013 11:51 AM