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Test Order

Mycobacterium tuberculosis Complex:
Drug Susceptibility Testing, First Line

Pre-Approval Required

None

Supplemental Information or Form

Form LAB 444 (03/12) (MDL-N-25), EXAMINATION FOR MYCOBACTERIUM TUBERCULOSIS DRUG SUSCEPTIBILITY

Performed on Specimens from

California local public health laboratories

Acceptable Sample/Specimen Type for Testing

Pure culture

Minimum Volume Required

1 ml if broth

Storage & Preservation of Specimen Prior to Shipping

Incubated at 35-37°C (or refrigerated).

Transport Medium

None

Specimen Labeling

Patient’s name, accession number, inoculation date or subculture date.

Shipping Instructions which Include Specimen Handling Requirements

Ship as infectious substance.

Test Methodology

By MGIT 960. Drugs routinely tested are isoniazid (INH) 0.1 μg/ml, rifampin 1 µg/ml, ethambutol 5 µg/ml, and pyrazinamide 100 µg/ml.  When there is resistance to any first-line drug, second-line drug susceptibility testing is performed.  In addition, when there is resistance to INH at 0.1 μg/ml, testing at  0.4 μg/ml is performed.

Turnaround Time

average 2 weeks

Interferences & Limitations

Results unattainable because culture submitted is not pure.

Additional Information

None

MDL Points of Contact

Mycobacterial Diseases Section (drug susceptibilities) (510) 412-3929 
Email: grace.lin@cdph.ca.gov

 
 
Last modified on: 8/26/2016 11:14 AM