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Test Order

Legionella Identification or Confirmation to Genus

Pre-Approval Required


Supplemental Information or Form

1.    Pure, viable subculture

2.    Completed submittal form with 2 patient identifiers (e.g. name and date of birth), collection date and source, a brief but complete patient history, cultural history and submitting laboratory’s findings

3.    Documentation that indicates the role of this isolate in clinical disease

Performed on Specimens from


Acceptable Sample/Specimen Type for Testing

Suspected Legionella sp. isolate

Minimum Volume Required


Storage & Preservation of Specimen Prior to Shipping


Transport Medium

Tubed agar medium with a secure screw cap, labeled with patient’s name.  Alternatively, blocks of BCYE agar may be aseptically cut from plates and then inserted into tubed agar medium of another type with a secured screw cap.

Specimen Labeling


Shipping Instructions which Include Specimen Handling Requirements

1.    Grow isolate under appropriate conditions prior to shipping.

2.    Ship at ambient temperature using appropriate DOT/IATA approved shipping procedures.

Test Methodology

As needed - Growth preference, Gene Sequencing by PCR, and/ or MALDI-TOF mass spectrometry

Turnaround Time

1 month

Interferences & Limitations

Mixed or nonviable culture

Additional Information

Please contact the MDL Special Pathogens Unit for special consideration if any of the above criteria cannot be met.

MDL Points of Contact

Special Pathogens Unit (510) 412-3903

Monday-Friday 8 AM to 5 PM PT

Emails: margot.graves@cdph.ca.gov or robin.hogue@cdph.ca.gov

Last modified on: 10/8/2013 11:38 AM