Supplemental Information or Form
1. Pure, viable subculture
2. Completed submittal form with 2 patient identifiers (e.g. name and date of birth), collection date and source, a brief but complete patient history, cultural history and submitting laboratory’s findings
3. Documentation that indicates the role of this isolate in clinical disease
Performed on Specimens from
Sputum, lower respiratory tract, less commonly pleural fluid, blood, and rarely various others
Acceptable Sample Type for Testing
A pure, viable subculture of a suspected Legionella sp. isolate
Minimum Volume Required
Storage & Preservation of Sample Prior to Shipping
Tubed agar medium with a secured screw cap. Alternatively, blocks of BCYE agar may be aseptically cut from plates and then inserted into tubed agar medium of another type with a secured screw cap.
Patient's name, date of subculture, and organism suspected
Shipping Instructions which Include Specimen Handling Requirements
1. Grow isolate under appropriate conditions and verify purity prior to shipping.
2. Ship at ambient temperature using appropriate DOT/IATA approved shipping procedures.
As needed - Growth preference, Gene Sequencing by PCR, and/ or MALDI-TOF mass spectrometry
Interferences & Limitations
Mixed or nonviable culture
Please contact the MDL Bacterial Diseases Section (Reference Bacteriology Unit) for special consideration if any of the above criteria cannot be met.
MDL Points of Contact
Reference Bacteriology Unit (510) 412-3903
Monday-Friday 8 AM to 5 PM PT
Emails: firstname.lastname@example.org or email@example.com
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