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Test Order

Bacterial Bioterrorism Agent
Identification or Confirmation

Pre-Approval Required


Supplemental Information or Form

1.    Pure, viable subculture

2.    For Brucella sp. confirmed to genus, a boiled undiluted cellular extract (see description below) of a pure culture

3.    Completed submittal form with 2 patient identifiers (e.g. name and date of birth), collection date and source, a brief but complete patient history, cultural history and submitting laboratory’s findings

Performed on Specimens from


Acceptable Sample/Specimen Type for Testing

1.    Bacterial isolate

2.    For Brucella sp.confirmed to genus, a boiled undiluted cellular lysate of a pure culture prepared using LRN extraction procedures with a final volume of 100 µl is also acceptable

Minimum Volume Required


Storage & Preservation of Specimen Prior to Shipping


Transport Medium

1.    Bacterial isolate: Tubed agar medium with a secure screw cap, labeled with patient’s name

2.    Boiled undiluted cellular lysate:  Minimum volume of 100 µl in a 1.5 to 2 ml taped screw cap conical centrifuge tube, labeled with the patient’s name

Specimen Labeling


Shipping Instructions which Include Specimen Handling Requirements

For Isolates:

1.    Grow isolate under appropriate conditions prior to shipping.

2.    Ship at ambient temperature using appropriate DOT/IATA approved shipping procedures.

For Cellular Extracts:

1.    Ship frozen or on a cold pack using appropriate DOT/IATA approved shipping procedures.

Test Methodology

As needed - Conventional Methods such as Biochemical testing, DFA, and phage lysis, and PCR

Turnaround Time


Interferences & Limitations

Mixed or nonviable culture

Additional Information

Please contact the MDL High Risk Pathogens Section for special consideration if any of the above criteria cannot be met.

MDL Points of Contact

High Risk Pathogens Section (Select Agents) (510) 412-3903
fax number (510) 412-3706

Emails: fengfeng@cdph.ca.gov or margot.graves@cdph.ca.gov

Last modified on: 1/7/2015 1:36 PM